According to the WHO about 80% population of the world rely on medicinal plants for remedies. Medicinal plants are exploited for therapeutic medicine and have been used by Ayurveda, Unani and Siddha. Portulaca grandiflora, is a medicinal ornamental plant in the family Portulacaceae, Portulaca has many medicinal properties against cirrhosis of the liver, pharyngeal pain and swelling, scurvy, snake and insect bites, burns, scalds, and eczema. In the present investigation complete plant regeneration was achieved only in single medium i.e. auxin (Indole butyric acid). Nodal explants of Portulaca grandiflora were inoculated on MS medium containing IBA (1.0-4.0mg/l). Axillary bud proliferation was observed within 05 days and root initiation was also observed on the same medium within 10 days of inoculation. No callus initiation was observed on any of the IBA supplemented media. After 3 weeks regenerated shoots attain height of 2-3 inch transferred to green house and finally to the field. Out of the four concentration used 3.0 mg/l IBA Axillary bud proliferation (86.12 %,) and plantlet regeneration (89.23). Regenerated plantlets were successfully transferred to green house and finally to field with 90% survival rete.
Keywords: Micropropagation, Portulaca grandiflora, Regeneration
Harisharan Goswami, Rampratap Singh